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Santa Cruz Biotechnology mdm2
Figure 2. Effect of carnosol on cellular markers of apoptosis in HCT116 cells. Cells were treated with indicated concentrations of carnosol for 24 h. (A) Immunoblot analysis for detecting the levels of cleaved caspase-9, and -3, and cleaved PARP. (B) After 24 h of incubation with carnosol (indicated concentrations) or etoposide (85 µM), the caspase-3 activity assay was performed as described in Materials and methods. (C) Western blot analysis of p53 and <t>Mdm2</t> expression. (D) The expression level of Bcl-2, Bcl-xl and Bax. Data are representative of three different experiments.
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Figure 2. Effect of carnosol on cellular markers of apoptosis in HCT116 cells. Cells were treated with indicated concentrations of carnosol for 24 h. (A) Immunoblot analysis for detecting the levels of cleaved caspase-9, and -3, and cleaved PARP. (B) After 24 h of incubation with carnosol (indicated concentrations) or etoposide (85 µM), the caspase-3 activity assay was performed as described in Materials and methods. (C) Western blot analysis of p53 and <t>Mdm2</t> expression. (D) The expression level of Bcl-2, Bcl-xl and Bax. Data are representative of three different experiments.
Primary Murine Antidextran Antibody No. 14533, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 2. Effect of carnosol on cellular markers of apoptosis in HCT116 cells. Cells were treated with indicated concentrations of carnosol for 24 h. (A) Immunoblot analysis for detecting the levels of cleaved caspase-9, and -3, and cleaved PARP. (B) After 24 h of incubation with carnosol (indicated concentrations) or etoposide (85 µM), the caspase-3 activity assay was performed as described in Materials and methods. (C) Western blot analysis of p53 and <t>Mdm2</t> expression. (D) The expression level of Bcl-2, Bcl-xl and Bax. Data are representative of three different experiments.
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Cayman Chemical anti-ip receptor (polyclonal-rabbit; 1:2,000)
Figure 2. Effect of carnosol on cellular markers of apoptosis in HCT116 cells. Cells were treated with indicated concentrations of carnosol for 24 h. (A) Immunoblot analysis for detecting the levels of cleaved caspase-9, and -3, and cleaved PARP. (B) After 24 h of incubation with carnosol (indicated concentrations) or etoposide (85 µM), the caspase-3 activity assay was performed as described in Materials and methods. (C) Western blot analysis of p53 and <t>Mdm2</t> expression. (D) The expression level of Bcl-2, Bcl-xl and Bax. Data are representative of three different experiments.
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Cell Signaling Technology Inc murine il 7
Figure 2. Effect of carnosol on cellular markers of apoptosis in HCT116 cells. Cells were treated with indicated concentrations of carnosol for 24 h. (A) Immunoblot analysis for detecting the levels of cleaved caspase-9, and -3, and cleaved PARP. (B) After 24 h of incubation with carnosol (indicated concentrations) or etoposide (85 µM), the caspase-3 activity assay was performed as described in Materials and methods. (C) Western blot analysis of p53 and <t>Mdm2</t> expression. (D) The expression level of Bcl-2, Bcl-xl and Bax. Data are representative of three different experiments.
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Innovagen AB igg1 humanized monoclonal antibody 11b6
Figure 2. Effect of carnosol on cellular markers of apoptosis in HCT116 cells. Cells were treated with indicated concentrations of carnosol for 24 h. (A) Immunoblot analysis for detecting the levels of cleaved caspase-9, and -3, and cleaved PARP. (B) After 24 h of incubation with carnosol (indicated concentrations) or etoposide (85 µM), the caspase-3 activity assay was performed as described in Materials and methods. (C) Western blot analysis of p53 and <t>Mdm2</t> expression. (D) The expression level of Bcl-2, Bcl-xl and Bax. Data are representative of three different experiments.
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Santa Cruz Biotechnology murine anti β actin loading control
Figure 2. Effect of carnosol on cellular markers of apoptosis in HCT116 cells. Cells were treated with indicated concentrations of carnosol for 24 h. (A) Immunoblot analysis for detecting the levels of cleaved caspase-9, and -3, and cleaved PARP. (B) After 24 h of incubation with carnosol (indicated concentrations) or etoposide (85 µM), the caspase-3 activity assay was performed as described in Materials and methods. (C) Western blot analysis of p53 and <t>Mdm2</t> expression. (D) The expression level of Bcl-2, Bcl-xl and Bax. Data are representative of three different experiments.
Murine Anti β Actin Loading Control, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 2. Effect of carnosol on cellular markers of apoptosis in HCT116 cells. Cells were treated with indicated concentrations of carnosol for 24 h. (A) Immunoblot analysis for detecting the levels of cleaved caspase-9, and -3, and cleaved PARP. (B) After 24 h of incubation with carnosol (indicated concentrations) or etoposide (85 µM), the caspase-3 activity assay was performed as described in Materials and methods. (C) Western blot analysis of p53 and Mdm2 expression. (D) The expression level of Bcl-2, Bcl-xl and Bax. Data are representative of three different experiments.

Journal: International journal of oncology

Article Title: Carnosol induces apoptosis through generation of ROS and inactivation of STAT3 signaling in human colon cancer HCT116 cells.

doi: 10.3892/ijo.2014.2281

Figure Lengend Snippet: Figure 2. Effect of carnosol on cellular markers of apoptosis in HCT116 cells. Cells were treated with indicated concentrations of carnosol for 24 h. (A) Immunoblot analysis for detecting the levels of cleaved caspase-9, and -3, and cleaved PARP. (B) After 24 h of incubation with carnosol (indicated concentrations) or etoposide (85 µM), the caspase-3 activity assay was performed as described in Materials and methods. (C) Western blot analysis of p53 and Mdm2 expression. (D) The expression level of Bcl-2, Bcl-xl and Bax. Data are representative of three different experiments.

Article Snippet: Primary antibody against each of p53, murine double minute-2 (Mdm2), Lamin-A, and horseradish peroxidase-conjugated secondary antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Techniques: Western Blot, Incubation, Caspase-3 Activity Assay, Expressing